From A260 to Actionable Data: Mastering Microvolume Spectrophotometry for Nucleic Acids
Essential principles of DNA and RNA quantification with microvolume measurements Accurate DNA and RNA quantification is foundational to molecular biology, driving success in workflows from qPCR and NGS to cloning and CRISPR screens. At the heart of routine quality control is absorbance at 260 nm, where nucleic acids exhibit a strong UV signal. The Beer–Lambert